|
KMID : 0613820170270121452
|
|
Journal of Life Science
2017 Volume.27 No. 12 p.1452 ~ p.1461
|
|
|
Black Sesame Ethanolic Extract Promotes Melanin Synthesis
|
|
Jeon So-Jeong
Kim Moon-Moo
|
|
|
Abstract
|
|
|
|
Melanin production by melanocytes in human hair follicles decreases with time and leads to the graying process, which is a phenotype of human aging and an index of aging. The reduction in melanin production is the result of decreased tyrosinase activity in hair follicles and an accumulation of active oxygen species, such as hydrogen peroxide. This study investigated antioxidant effects and melanin- promoting effects in B16F1 cells treated with black sesame ethanolic nonpolar-soluble extract (SBEEO) and black sesame ethanolic polar-soluble extract (SBEEP). In antioxidation experiments, both SBEEP and SBEEO did not eliminate 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, but SBEEO at 64 μg/ml showed low reducing power. SBEEP exerted cytotoxic effects at concentrations greater than 8 μg/ml, whereas SBEEO showed cytotoxic effects at concentrations greater than 4 μg/ml. SBEEP and SBEEO induced melanin synthesis, tyrosinase activity, and DOPA oxidase activity in vitro. In live cells, melanin synthesis was greater in the SBEEP treatment group as compared with that in the SBEEO treatment group. SBEEP stimulated melanin synthesis by modulating the expression of tyrosinase-related protein-2 (TRP-2), which is an important enzyme in melanin synthesis. These results imply that SBEEP obtained from black sesame ethanolic extract may have the potential to improve melanin synthesis.
|
|
|
KEYWORD
|
|
|
Black sesame, melanin, tyrosinase, TRP-1, TRP-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|